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一.產品名稱：Recombinant Rat TPO

二.Catalog Number:AF-400-34

三.Synonyms:

Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF

四.Description:

TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of plaets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating plaets. Human and murine TPO exhibits cross-species reactivity. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein. Manufactured using all non-animal reagents.

五.AA Sequence:

SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF

六.Source:E.coli

七.Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.

八.Biological Activity:

The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁶ units/mg.